Entrepreneurship education in higher education requires strategies that connect theory with practice. Although Project-Based Learning (PBL) is widely used, less is known about the educational value of interactive guides that support students throughout business-plan development. This study examined a Genially-based instructional guide, supplemented with YouTube multimedia, within a PBL intervention in the Business Innovation and Creativity course at Universidad Veracruzana. A quantitatively driven embedded mixed-methods, one-group pre–post design was used with 28 undergraduate students. Data came from course assessment records and pre- and post-intervention questionnaires. Quantitative analysis compared group-level response patterns across 23 conceptually aligned dichotomous items on self-reported entrepreneurship and business-planning knowledge, while open-ended responses explored students’ perceptions of the learning experience. Descriptive statistics and thematic analysis were conducted, and both strands were integrated during interpretation. Post-intervention affirmative-response percentages were higher for several conceptually aligned topics, although the response patterns varied across items and the anonymous responses could not be matched at the individual level. Students also rated the guide as highly useful (M = 9.81/10) and described it as providing organization, clarity, and step-by-step support. These findings suggest that the guide had potential educational value as a pedagogical scaffold in this course. However, because the study used a small, one-group design without linked responses or a comparison group, the results should be interpreted as context-specific descriptive evidence rather than as proof of causal effects on entrepreneurial competencies.