Academic studies are an important stage in the development of professional identity and perceptions regarding a person’s future occupation. This study examined the relationships between experiences during college studies and professional identity and motivation to work in the studied field among female undergraduates from the Ultra-Orthodox sector. The study employed a cross-sectional survey design with a sample of 93 participants, who were all studying for B.A. degrees on an Ultra-Orthodox college campus. At the end of the 2023 academic year, participants completed anonymous, self-report questionnaires about their attitudes toward academic studies, professional identity, and motivation to work. The data from the questionnaires were analyzed using t-tests, ANOVA, correlation analyses, and multiple regression. The findings revealed significant, positive relationships between satisfaction with one’s studies and professional identity and between professional identity and motivation to work in one’s field of study. Contrary to what had been hypothesized, no relationship was found between the students’ satisfaction with their studies and their motivation to work in their field of study. In addition, the professional identity of students in the later years of their programs was no stronger than that of first-year students. This indicates that perceptions of one’s college studies are not directly related to occupation or motivation for work. The findings show that the year in one’s study program did not affect work motivation, suggesting that the link between academic experience and occupation is weak. In addition, the fact that professional identity did not act as a mediating factor in the examined model suggests that professional identity may develop before individuals begin their college studies.